What Is Actin Antibody?

One of the six known forms of actin, beta-actin, has been identified. Beta-actin, a non-muscle, cytoskeletal protein, is found in all cell types. It is involved in cell structure, motility, and integrity. Six different forms of actin are found in humans. Gamma and beta actin are both present in all cell types. All eukaryotic cells contain beta actins, which are cytoplasmic protein abundantly expressed. 

The polymerization of G-actin (globular actin) results in a structural filament (F -actin), which is composed of two stranded Helixes. Actin antibodies are highly conserved proteins, are important for cell structure, motility and integrity. Beta-actin is found in all eukaryotic cell types and is often used as a loading control for protein detection assays such as Western Blotting. 

Antibodies against beta-actin are a useful tool for studying the intracellular beta-actin distribution and dynamic aspects. Monoclonal anti-beta actin antibody can be used for the analysis of beta-actin expression in experimental cells using immunofluorescence or western blot. 

The antibody specifically recognizes beta-actin in a wide variety of tissues and species using immunoblotting, immunofluorescent staining of cultured cell lines, and immunohistochemistry.  The antibody recognizes an epitope that is insensitive to formalin-fixation or paraffin-embedding. You can also use Bouin's solutions, B5, methacarn or Ethanol as fixatives.

ACTB (actin b), gene codes for b-actin. It is a cytoskeletal housekeeping protein. It can be found in the cytoplasm. This gene can be found on the human chromosome 7p22.1. Recognizes an epitope at the N-terminal of the b -isoform actin. It labels b-actin in a variety of tissues and species. The antibody is used to label the dense bodies and longitudinal channels that link consecutive dense bodies.